ANCA
C-ANCA
P-ANCA
Anti-Neutrophil Cytoplasmic Antibodies (ANCA) are detected by immunofluorescence assays in more than 95% of vasculitis patients. ANCA are autoantibodies that react with neutrophil specific antigens, most frequently myeloperoxidase (MPO) and proteinase-3 (PR3).
On ethanol-fixed neutrophils two prominent patterns of ANCA can be identified, a diffuse, granular cytoplasmic staining (C-ANCA) and a perinuclear staining with or without nuclear involvement (P-ANCA). Less frequently, atypical ANCA patterns not fitting the descriptions of C and P-ANCA can be recognised.
Using a combination of ethanol-fixed neutrophils and formalin-fixed neutrophils can help to distinguish non-ANCA artefacts from true P-ANCA and C-ANCA staining patterns.
Binding Site offers neutrophil slides and kits fixed with both ethanol and formalin. These are ideal for ANCA screening. For confirmation of specificity refer to the proposed ANCA testing protocol and the MPO and PR3 BINDAZYME kits.
| Description | Wells | Code |
|---|---|---|
| ANCA COMBI kit | 10 x 5 | FK018 |
| ANCA ethanol kit | 10 x 5 25 x 10 | FK016 FK016.1 |
| ANCA formalin kit | 10 x 5 | FK017 |
| Description | Wells | Code |
|---|---|---|
| Ethanol-fixed neutrophil slide | 10 x 5 25 x 10 | FS016.1 FS016.2 |
| Formalin-fixed neutrophil slide | 10 x 5 25 x 10 | FS017.1 FS017.2 |
| Affinity purified anti human IgG (H&L) with Evans Blue (Prediluted for ANCA) | 12.5mL 250 test | FA003.A
|
| C-ANCA positive control serum P-ANCA positive control serum | 0.5mL 0.5mL | FA136 FA130 |
| Neutrophil mounting medium | 10mL 100mL | CON46.2 CON46.3 |
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