ASCA IgA Kit

This assay is intended as an aid in the diagnosis of inflammatory bowel disease (IBD), specifically Crohn's disease. The kit is designed for the detection and quantitation of IgA anti-Saccharomyces cerevisiae antibodies in human serum.

Antigen

The microwells are precoated with mannan isolated from Saccharomyces cerevisiae.

Calibration

The assay is calibrated to U/mL against an arbitrary internal reference calibrator, as no internationally recognised reference preparation is currently available. A 5 point calibration curve is generated. A cut-off control is provided if being used as a screening assay.

Result Interpretation

Quantitative Assay

Result	Interpretation
< 7.0 U/mL	Negative
> 7.0 U/mL	Positive

Screening Assay

Result	Interpretation	Action
OD greater than cut-off control	Suspected of having ASCA	Test in the quantitative assay if value required
OD less than or equal to cut-off control	Negative	Report as negative

Result

Interpretation

Action

OD greater than cut-off control

Suspected of having ASCA

Test in the quantitative assay

if value required

OD less than or equal to cut-off control

Negative

Report as negative

Measuring Range

1.23 - 100 U/mL

Sensitivity

0.93 U/mL