SPAplus® Automated Analyzer for Specialist Protein Analysis
The SPAplus® is a fully automated, high throughput, easy-to-use, table top, turbidimetric protein analyzer for serum, plasma, and urine assays including Freelite®, IgG subclasses, IgA subclasses, IgG, IgA, IgM, IgD, Cystatin C, C3, C4, β2 Microglobulin, Albumin, Haptoglobin, Prealbumin, and an expanding range of new assays under development.

The flexibility and versatility of the SPAplus® System and its compact design make it an ideal companion analyzer to your automated chemistry line as well as your special chemistry testing.

Read what our customers have experienced:


“We have been utilizing the SPAplus® analyzer for over 4 years now. We find the system robust and have had very few problems with it. The technical staff are comfortable operating the instrument and find it efficient and easy to use. The pro-zone detection is excellent and the measuring range for serum free light chains very satisfactory. Very few off-line dilutions are necessary. Our clinicians are pleased with our ability to offer serum free light chains to help them manage their patients and we have seen a decrease in the volume of urine protein electrophoresis requests.

We would recommend the 
SPAplus® analyzer to any laboratory interested in offering serum free light chains in the diagnosis and monitoring of plasma cell dyscrasias.”


"We have been using the SPAplus® for two years. Since using the SPAPLUS it has saved us time and energy running free light chains (kappa and lambda), and we have added another assay, Beta-2 Microglobulin. Daily maintenance is minimal, and the auto antigen excess check is wonderful, saving us time by performing most of the necessary dilution for us. The results are rapid, only 10-15 minutes, and the monitor screen on the CPU gives real-time analysis data. More tests are in the pipeline for FDA approval on the SPAplus, such as CH50 and Hevylite®, which we may add in the near future and thus continue to save us tech time. Overall, the SPAplus® is a very robust instrument, and hopefully its software can soon catch up with it in the next version update.”


“I’ve been very happy with this analyzer with its ease of operation, user-friendly software, minimal maintenance, accuracy, reliability, dependability, and I could go on and on. Overall, Dr. Glenn Tisman and I are very satisfied customers.

I am looking forward to getting the prealbumin, haptoglobin, and immunoglobulins validated as soon as possible. There is no doubt in my mind that your Technical Support crew’s expertise will get the validation done in no time. They have been very helpful and reliable all these years.

Again, your professionalism and exceptional services are very much appreciated.”

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“The SPAplus® was the perfect size to place next to our electrophoresis bench. Now we have a specific technologist performing ELP & FLC side-by-side. It has helped us create a workflow with the convenience of these related tests being performed next to each other.”

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“The SPAplus® has provided reliable, accurate results for serum free light chains in our laboratory. The positive impact of its reliability on patient care has been important to our clinicians and their practice, while Binding Site has provided excellent service and support for this product. We look forward to implementing additional testing on the SPAplus in the near future.”

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“We installed and validated the SPAplus® Analyzer here at CPL--Austin main laboratory to replace the Siemens BN-II for Serum Free Light Chain assays. The system has performed beautifully with little or no down time since it went on line. The technical staff testify that it is a great improvement for our laboratory with several qualities including:

·         Improved overall efficiency
·         Ease of use
·         Faster overall through-put
·         Less sample volume required
·         No “pro-zone” issues with the system's antigen access checking

Our clients are happy, and our volume has increased since we brought this assay in-house. We would highly recommend this system to other laboratories for these assays.” M. Callaway, M.T.(ASCP)

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"The SPAplus® has been able to handle smaller pipetting volumes, and it has provided greater consistency in the reporting of the serum free light chain results than our previous platform, the IMMAGE.

The serum free light chain ratio offers the best opportunity for "real time" monitoring of many patients with serum paraproteins given the relatively short half-life of a free light chain versus a complete paraprotein.

In addition, the increased sensitivity has enhanced detection of plasma cell neoplasms such as plasma cell myeloma but also some lymphoproliferative disorders, especially those that might go undetected because of a negative serum protein electrophoresis and or serum immunofixation study.  

The serum free light chain study is a truly quantitative study, and it is generally easier to interpret than serum protein electrophoresis and serum immunofixation studies.

In most instances the serum free light chain test has obviated the need for urine protein electrophoresis and urine immunofixation studies.” L. Johnson, MD, FCAP, FASCP




Rapid results: Time to first result in just 15 minutes; subsequent results every 30 seconds.

Auto-dilution: Identifies samples that are outside the measuring range, and automatically runs them at a higher to lower dilution, as required.

Ready-to-Use barcoded reagents: Eliminate the need for pouring into secondary containers. Easy automatic ID and registration.

On-board reagent cooling: Ensures reagent stability without the need to transfer reagents in and out of the refrigerator.

Intelligent cuvette wash system: 10-step acid/alkali/hot water wash results in excellent precision and assay performance, especially for latex assays.

Automatic cuvette monitoring: Measures the blank optical density of each individual cuvette indicating when it is time to change them.

Automatic antigen excess (prozone) check: Monitors the reaction kinetics and runs that sample at a higher dilution when reaction profile is indicative of antigen excess, for applicable assays.

Air mixing: Eliminates carry-over.

Easy loading and unloading with primary tube sample tray.

Intuitive Windows®-based software makes it easy to set up the run and walk away.

Fully integrate bi-directional RS232 interface capability.

Minimal hands-on daily maintenance (less than 10 minutes per day) allows quick and easy start up and shut down.

Regional Medical Laboratory in Tulsa, Oklahoma uses SPAPLUS to run its Freelite® assays.

"The serum free light chain study is a truly quantitative study, and it is generally easier to interpret than serum protein electrophoresis and serum immunofixation studies.

In most instances the serum free light chain test has obviated the need for urine protein electrophoresis and urine immunofixation studies.

The SPA has been able to handle smaller pipetting volumes, and it has provided greater consistency in the reporting of the serum free light chain results than our previous platform."

Lawrence R. Johnson, MD, FCAP, FASCP
Regional Medical Laboratory
Tulsa, Oklahoma




  • Incubation time: 10.5 minutes
  • Time to first result: 15 minutes, and each consecutive sample thereafter – 30 seconds
  • 240 tests per hour (up to 120 tests/hour with sample dilution)

Reagent Handling

  • 24 reagent positions
  • 30-day on-board reagent stability for most assays (28 days for IgD, 40 days for Freelite®)
  • Onboard reagent cooling 

Sampling Handling

  • Sample Type: serum, plasma, urine (β2M)
  • 40 samples, 30 with barcodes per rack
  • Auto-dilution capability
  • Sample containers: primary and secondary tubes and sample cups
  • Sample reaction volume (aspirated): 3-30µL
  • Minimum sample volume (dead volume in cup or elevated 12x75mm tube): 150 µL
  • Integral barcode reader (18 digits max), accepts the following barcodes: Code 39, Code 128, ITF, NW-7


  • Antigen Excess (Prozone) detection
  • Automated high temperature cuvette washing system
  • 60 semi-permanent acrylic cuvettes
  • Air pressure mixing
  • Automatic interior/exterior probe washing between reagent and/or sample addition
  • Reagent and sample level detection
  • 12 wavelengths (340 – 800 nm)
  • Tungsten Halogen lamp (~2000 hrs)


  • Dimension: 31.5"/800mm (Width) x 25.2"/640mm (Depth) x 20.5"/520mm (Height)
  • Weight: 210 lbs / 96kgs
  • Power requirements: AC 100/115/230 V 50/60 Hz
  • Rated power consumption: 600 VA
  • Peltier reagent cooling: 10°C +/- 2°C
  • Diluted acid and alkaline wash bottles (3L each) at side of instrument
  • Water and waste (10L each), and spare water and waste containers (5L each) below the instrument

Maintenance / Service  

  • Daily maintenance less than 10 minutes hands-on
  • Monthly maintenance less than 15 minutes hands-on
  • 1-year limited service warranty
  • 24/7 toll free assistance

The SPAPLUS® System offers an expanding menu of diagnostic tests related to disease state management and clinical practice guidelines.

Freelite® Kappa - Free Kappa Light Chain
Freelite® Lambda - Free Lambda Light Chain
Albumin CSF 60 NK032.L.S.
Alpha-1-Acid Glycoprotein 100
Alpha-1-Antitrypsin 100 NK034.S
Alpha-2-Macroglobulin 100 NK039.S
Anti-Streptolysin O 100
Apolipoprotein A-1 100 NK085.S
Apolipoprotein B 100 NK086.S
Beta-2-Microglobulin 100
Beta-2-Microglobulin Urine 100 LK043.U.S.
C1 Inactivator 100
C3c 100
C4 100 NK025.S
Ceruloplasmin 50 NK045.S
CH50 200
        - Controls (CH50) 4 sets NQ095.S
        - Calibrators (CH50) 1 set NC095.S
Cystatin C 100
Haptoglobin 100
IgA 100
IgA1 50
IgA2 50
IgA CSF 60
IgD 100
IgG 100
IgG1 100
IgG2 100
IgG3 100
IgG4 100
IgG CSF 60
IgM 100
IgM CSF 60
Lipoprotein (a) 100 LK098.S
Prealbumin 100
Transferrin 100
T.Tox Plasma Screen Only§ 1000




Clinical Assays in Development:

  • Full Range C-Reactive Protein*
  • Ferritin*
  • Hevylite® IgA Kappa*
  • Hevylite® IgA Lambda*
  • Hevylite® IgG Kappa*
  • Hevylite® IgG Lambda*
  • Hevylite® IgM Kappa*
  • Hevylite® IgM Lambda*
  • IgE≈
  • Microalbumin*
  • Rheumatoid Factor*


        *    Pending FDA Clearance

        ≈   In Development

        §   RUO



Please contact us for more information about our products and services.


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